Asclepiadaceae a wildly growing plant has been reported to possess number of medicinal properties and other purposes. To investigate the effects of calotropis gigantea root bark on wound healing activity in rats by excision. Antioxidant, antimicrobial activities and gcms analysis. A downloadable pdf document of a book in prepublication awaiting illustration. Antimicrobial activity and phytochemical analysis of calotropis gigantea root, latex extracts. Antimicrobial activity of ethanol, methanol and chloroform extracts from flower, leaves and roots of c. Calotropis gigantea is a well known indian medicinal plant, distributed in most parts of india. Shahzaib, abdullah khan, arif waqar zaif, wasim ahmed department of biotechnology, university of science and technology bannu, khyber pakhtunkhwa pakistan key words. Traditionally calotropis is used alone or with other medicinals caius 1986 to treat common. They were kept at 2225 c, 12 h lightdark cycle and had free access to food and water.
Antibacterial, calotropis, leaves, microscopical, phytochemical. Calotropis gigantea is usedtraditionally to treat common diseases such as fever, rheumatism, indigestion, cough, cold, eczema, asthma, elephantiasis, nausea, vomiting and diarrhoea, either alone or with other medicinesaim of the study. Antifungal, antioxidant and antibacterial activities of calotropis procera mushtaq ahmed, rehmat ali khan, s. Preliminary phytochemical screening of calotropis gigantea leaf suchita singh, sanchita singh, r. The plant is erect, tall, large, much branched and perennial with milky latex throughout. Single step green synthesis of stable nickel and nickel oxide nanoparticles from calotropis gigantea. Pg and research department of microbiology, selvamm arts and science college, namakkal, tamil nadu, india. Antimicrobial potential of calotropis gigantea against a wide range of microorganisms was studied.
Both of methanol extract and its chloroform fraction showed activity against sarcina lutea, bacillus megaterium. Pdf the antimicrobial activity of crude aqueous extract of latex of calotropis gigantea apocynaceae was tested against six pathogenic species of. To evaluate the antivenom activity of calotropis gigantea plantextract against vipera russelli snake venom. Green synthesis of silver nanoparticles using calotropis. Calotropis gigantea belonging to the family asclepiadaceae has been investigated for the presence of its extracts with special emphasis to the brine shrimp lethality bioassay, thrombolytic activity and antimicrobial activity.
The latex of calotropis gigantea contains cardio glycosides, volatile fatty acids and calcium oxalate. Calotropis gigantea crown flower is a species of calotropis native to cambodia, indonesia, malaysia, philippines, thailand, sri lanka, india and china. Rizvi2 1department of pharmacology, bundelkhand university, jhansi, india, 284128. Flow cytometric evaluation of calotropis gigantea for.
Although calotropis gigantea have various ethnopharmacological uses, the plant have not been undergone any chemical and pharmacological investigation. Medicinal plants are having immense potential to cure various health ailments and used as drugs and remedies for the treatment of various diseases since civilization. An excellent, if rather terse, guide to the traditional medicinal uses of the plants of the region in nepal, the latex is applied to sprains, body pains, boils and pimples. The objective of present work was to find the bioactive constituents present in the ethanolic leaf extract of c. Small trees or shrubs, these two species are native to southern asia and africa and were introduced to south america and the islands of the caribbean, where they have naturalized. Antimicrobial screening and brine shrimp lethality. The present study was aimed to investigate the phytochemical screening and antimicrobial activities of calotropis gigantea leaf extracts. Maximum activity was seen in case of pseudomonas fluorescens where the zone diameter was 32 mm 300. Calotropis procera, latex, antimicrobial, antioxidant, antifungal. Growing calotropis gigantea in the villages, fl zone 9a. Antimicrobial activity of latex of calotropis gigantea against pathogenic microorganisms an in vitro study gaurav kumar, loganathan karthik and kokati venkat bhaskara rao molecular and microbiology research lab, environmental biotechnology division, school of bio science and technology, vit university.
However there are no scientific reports on wound healing activity of the plant calotropis gigantea r. Antibacterial activity of agnps was evaluated against gram positive and gram negative human pathogens using agar well diffusion method. The ethyl acetate extract of this plant showed significant showed significant antibacterial and antifungal effect against most of the pathogenic organic organisms. Antimicrobial activity of flower extracts of calotropis. The antimicrobial effect of ethanol, aqueous and chloroform extracts of leaf and latex of calotropis procera on six bacteria namely, escherichia coli.
Pharmacological studies on aerial parts of calotropis procera. The structures of these compounds were confirmed by spectroscopic analyses ir, hrtofms and nmr. Evaluation of antibacterial activity of calotropis gigantea latex extract on selected pathogenic bacteria. Collection of calotropis gigantea the fresh, young disease free herbal plant. Invitro antimicrobial activities and phytochemical. Cns activity of calotropis gigantea roots sciencedirect. A phytoehemieal study on the flowe r of calotropis gigantea linn. Study on antibacterial activity of calotropis procera. University rewa india nbri lucknow india abstract herbals plants are effective source of traditional and modern medicines, useful for primary health care. Dec 03, 2010 the hypoglycemic activity in normal rats was carried out by treatment using chloroform extract of calotropis gigantea leaf and flower 10, 20 and 50 mgkg, orally. Antimicrobial activity and phytochemical analysis of calotropis. Phytochemical and pharmacological investigation of.
Calotropis is used as a traditional medicinal plant with unique properties. Pdf the antibacterial activity of methanol extract from the root bark of akanda calotropis gigantea l. It has been found that the light radiation affects the antimicrobial activity of herbal products. Insecticidal activity of root bark of calotropis gigantea l. Pattnaik pk1, kar d2, chhatoi h1, shahbazi s3, ghosh g4, kuanar a2. Calotropis gigantea linn and its chloroform and petroleum ether 4060c soluble fractions were evaluated against several inster of larvae and adult of tribolium castaneum. Antioxidant activity of crude extract and latex inhibition ranging from 1220mm while latex was found extracted from the calotropis procera more active which showed activity with inhibition zone fig. Calotropis procera extracts against seedborne fungi agrees with the work of abdulmoniem et al. From the results it was evident that the flower of vinca rosea showed the highest antioxidant activity of 97.
Antioxidant activity of calotropis gigantea by dpph method antioxidant activity concentrati in % on gml root extract ascorbic acid 100 200 300 82. Antioxidant, antimicrobial activities and gcms analysis of. Pdf antimicrobial activity of akanda calotropis gigantea l. The plant has oval, light green leaves and milky stem. Phytochemical analysis, antibacterial and antioxidant. Antioxidant, antimicrobial activities and gcms analysis of calotropis gigantea white flowers shopna rajamohan, prabakaran kalaivanan, ilayaraja sivagnanam, manivannan rajamanickam abstract calotropis gigantea white asclepiadaceae, is a weed plant commonly known as giant milkweed. It is a very common herb seen widely throughout india. Antimicrobial activity of akanda calotropis gigantea l. Maximum activity was conferred against eschershia coli 4mm and shigella 3. Anthelmintic activity the anthelmintic activity of calotropis procera linn. Insecticidal activity of calotropis procera extracts on the flesh fly, sarcophaga haemorrhoidalis fallen. To test of antimicrobial activity, disc diffusion method has been followed. In this study, using the illumina high throughput sequencing. Therefore the present study was designed to investigate antimicrobial and cytotoxic activity leave extract of calotropis gigantean.
Antibacterial and phytochemical analysis of stem and root. Antimicrobial activity and phytochemical analysis of. Sep 22, 2015 the sodom apple calotropis gigantea, a member of the asclepiadaceae family, is a large evergreen shrub native to continental asia and northern africa. Antimicrobial and cytotoxic activity of di 2ethyl hexyl phthalate and anhydrosophoradiol 3acetate isolated from calotropis gigantea linn. Significant activity was shown by calotropis gigantea doses as compared to control. The leaves are broadly elliptical to oblongobovate in shape, with the size of 920 cm x 612.
World journal of pharmaceutical and life sciences wjpls. Calotropis gigantea is a common wasteland weed and known for various medicinal properties. Approximately 850 gm of the powder of whole plant of calotropis gigantea was soaked with ethanol and. Calotropis gigantea grows to a height of 8 to 10 ft 2. Pdf antimicrobial activity of akanda calotropis gigantea. Preliminary phytochemical, antimicrobial and photochemical. It has clusters of waxy flowers that are either white or lavender in colour. Nehru e journal research article a journal of nehru arts. This plant is popularly known because it produces large quantity of latex 1. Antimicrobial and cytotoxic activity of di2ethylhexyl. Evaluation of anti microbial activity and phytochemical. Flowers, in comparison with levamisole, was evaluated in a series of in vitro and in vivo studies.
In 1980, pal and sinha had isolated, crystallized and studied the properties of calotropins d1 and d2 from c. Medicinally important parts of this plant are flower, terminal leaf pairs, root with root bark and latex. Saini download pdf international journal of pharmaceutical and phytopharmacological research. Scientific investigations of medicinal plants have initiated in. The 11 analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and 12 flower of calotropis procera was carried out in disc method and also determined mic value at 600nm through optical density using spectrophotometer.
Purification and properties of sodom apple latex proteinases. Preliminary phytochemical screening and gc ms issn. Antimicrobial activity of latex of calotropis gigantea against pathogenic microorganisms an in vitro study article pdf available in pharmacologyonline 3. The antibacterial activity therefore shows clearly that the resistance to the pathogens may be minimized by these natural products of the plant origins. Rn a h tcol eg f p rm cy,s ns mhowneemuch road, mandsaur m. As a continuation, the chemical constituents present in ethyl acetate extract of calotropis gigantea l. Journal of nanoscience nanoengineering and applications.
Plant is one of the important source of drugs thats why we have done this project work to find out some biological activities of the plant exudates of calotropis gigantea where antimicrobial, cytotoxic and antioxidant activity were analyzed. The antibacterial activity of methanol extract from the root bark of akanda calotropis gigantea l. Calotropis gigantea, the crown flower, is a species of calotropis native to cambodia, indonesia, malaysia, the philippines, thailand, sri lanka, india, china, pakistan, nepal, and tropical africa. Antibacterial activity well plate method was employed on leaf extract of calotropis gigantea against certain gram positive b. Citeseerx document details isaac councill, lee giles, pradeep teregowda. Chemical characterization and insecticidal activity of. The purpose of the present study was to evaluate scientifically the antidiarrhoeal effects of c. In this report, we provide new information on the antimicrobial activities of c. Calotropis gigantea large milkweed leaves for caterpillars. Journal of the egyptian society of parasitology, 272. Pdf antimicrobial activity of latex of calotropis gigantea against. Out of that the most significant part which drew attention of researchers is latex because of its medicinal properties. Study of methanolic extract of leaves calotropis gigantea. Research article studies on anticonvulsant activity of stem.
Mudarol, the chief resinol component of the root bark ofcalotropis gigantea, has been separated into two isomeric compounds by fractional crystallisation of the acetates and also by chromatographic adsorption analysis of the free alcohols. This study attempts to determine the phytochemical analysis and antimicrobial effect of c. Br asclepiadaceae is a xerophytic, erect shrub, growing widely throughout the tropical and subtropical regions of asia and africa. The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity against clinical isolates of bacteria. Wound healing activity of calotropis gigantea root bark in rats.
Antimicrobial activities of calotropis procera on selected. The results obtained in these studies demonstrate unequivocally that like phenytoin and possessed anticonvulsant activity. Antimicrobial activity of latex of calotropis gigantea against pathogenic. Preliminary phytochemical screening of calotropis gigantea. Studies on antidiarrheal activity of calotropis gigantea r. The structures of these compounds were confirmed by spectroscopic analyses ir, hrtofms and. Calotropis belongs to asclepiadaceae or milkweed or ak family which includes 280 genera and 2,000 species of worldwide distribution but most abundant in the subtropics and tropics, and rare in cold countries. For anticonvulsant activity only male rats were used. Review article pharmacognostical aspects and pharmacological. Antimicrobial activity of flower extracts of calotropis gigantea. The aim of this investigation was to carry out the analysis of antibacterial and antifungal activities of different parts of calotropis gigantea.
Calotropis gigantea, flowers, gcms analysis, antioxidant, antimicrobial. Antifungal, antioxidant and antibacterial activities of. Antimicrobial activities of different parts of calotropis. But the 200 mg kg dose of ethanolic extract calotropis gigantea showed maximum anti inflammatory effect and was comparable to that produced by 100mg kg of. Giganteol which was first obtained from the stem bark is one of them and a new triterpene alcohol, isogiganteol is the other. The highest activity diameter of zone of inhibition 26 mm was demonstrated by the ethanolic extract of c. Calotropis gigantea arka uses, dose, side effects, ayurveda. Albino rats of either sex weighing between 100 and 150 g were selected. Bacillus subtilis mtcc 121, staphylococcus aureus mtcc 96, pseudomonas aeruginosa mtcc. A phytochemical study on the flowe r of calotropis gigantea linn. The in vitro studies demonstrated the anthelmintic effects p of crude aqueous cae and crude methanolic extracts cme of calotropis procera. A study on phytochemical screening and invitro antioxidant.
The different parts of calotropis gigantea and vinca rosea belonging to the families of asclepiadaceae and apocynaceae were studied for their antioxidant and antimicrobial activities against selected bacterial strains. Its a statuesque plant that likes part shade, sandy soil, and fairly dry conditions. Antibacterial activity of aqueous extract of calotropis. Antimicrobial activities evaluated from leaves, flowers, fruits and latex of locally obtained calotropis procera from alsharifieya, taif in different aqueous solutions and solvents such as sterilized zamzam water and distilled water, ethanol, acetone. The aim of the present study is to determine the antibacterial activity of silver nanoparticles synthesized using c. Preliminary phytochemical screening of calotropis gigantea leaf. The green fruits contain a toxic milky sap that is extremely bitter and turns into a gluey coating which is resistant to soap. Calotropis gigantea linn is popularly known as the swallowwort or milkweed and is used as one of the most important drug in traditional system of medicine to treat various ailments. As vicki from tampa writes, cats like tropical milkweed and native milkweed better than the tough leaves of calotropis, but ill continue to grow it for its beauty. Asclepiadaceae, commonly known as milkweed or swallowwort, is a common wasteland weed singh et al. Calotropis procera is a species of flowering plant in the family apocynaceae that is native to north africa, tropical africa, western asia, south asia, and indochina. It is widely used in many ayurvedic treatments, both externally ksharasutra and internally. Calotropis gigantea latex, antifungal activity, minimum fungicidal concentration, zone of inhibition, disc diffusion method correspondence to author. Antiinflammatory activity of calotropis gigantea and tridax procumbens on carrageenininduced paw edema in rats awasthi s.
Screening and gcms profiling of ethanolic flower extract of calotropis gigantea linn. Preliminary phytochemical screening and gc ms issn profiling. As an important medicinal shrub and a fiber resource plant, there is an urgent need for developing molecular markers to facilitate breeding and genetic improvement of varieties. The aim of the present study was to screen leaves of calotropis gigantea for the antimicrobial activity. Antibacterial activity of crude extract and latex crude was 20. Pdf antimicrobial activity and phytochemical analysis of. Calotropis gigantea linn apocynaceae is a glabrous or hoary, laticiferous shrubs or small trees, about 34 m tall commonly known as the swallowwort or milkweed. Approximately one fifth the activity of phenytoin but the activity lasted for 24 hrs. The first species being abundant while the second being restricted to forest areas. Asclepiadaceae a wildly growing plant has been reported to possess number of medicinal properties 8 and other purposes 9. The structures of these compounds were confirmed on the basis of ir, mass and nmr spectroscopy, and their cytotoxic activity against a431 human vulvalderived epidermoid carcinoma cell line was examined. Chitme hr, chandra r, kaushik s 2004 studies on antidiarrhoeal activity of calotropis gigantea r. Antifertility activity the effect of an ethanolic extract of the roots of calotropis procera has been studied in albino rats to explore its antifertility and hormonal activities.
Chemical composition of calotropis gigantea springerlink. The results of the in vitro antifungal assay revealed that the growths of fungal strains were affected by the latex extract by forming clear. Antimicrobial activities evaluation from the extracts of. Stem barks of calotropis gigantea had a slower onset of action and lesser degree of anticonvulsant activity. Screening of preliminary phytochemical analysis and. Other articles where calotropis gigantea is discussed. Calotropis gigantea crude extract, phytochemicals, antimicrobial activity. Phytochemical and pharmacological studies of the whole. Antiinflammatory activity of calotropis gigantea and tridax. Screening of preliminary phytochemical analysis and antibacterial activity of calotropis gigantea l. The root and latex of calotropis gigantea were screened for its antimicrobial and phytochemical activities. Jun 15, 2006 the alcoholic extract of calotropis gigantea was suspended in vehicle 5% tween 80.
Preliminary phytochemical screening and gcms profiling of. Anhydrosophoradiol3acetate, antimicrobial, calotropis gigantea and di2ethylhexyl phthalate the emergence of human pathogenic microorganisms that are resistant to major classes of antibiotics has been increased in recent years, due to the indiscriminate use of antimicrobial drugs karaman et al. Calotropis procera possesses antinociceptive activity, which is independent of the opioid system s aber et al. In residual film toxicity, methanol extract and its chloroform and petroleum ether fractions showed insecticidal activity against t. The leaves are sessile and subsessile, opposite, ovate, cordate at the base. Chandrabhan s, sumint st 2011 antibacterial efficacy and phytochemical analysis of organic solvent extracts of calotropis gigantean. Wound healing activity of latex of calotropis gigantea narendra nalwaya1, gaurav pokharna1, lokesh deb2, naveen kumar jain1 phone no.
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